KEANEKARAGAMAN DAN KARAKTERISASI FISIOLOGIS BAKTERI RIZOSFER TANAMAN NANAS (Ananas comosus L. MERR) DI DESA RIMBO PANJANG, KAMPAR, RIAU

Diversity and Physiological Characterization of Rhizosphere Bacteria from Pineapple (Ananas comosus L. Merr) Cultivation Areas in Rimbo Panjang, Kampar, Riau

Abstrak

Rhizosphere bacteria play a significant role in enhancing plant growth and health through nutrient cycling and biological control mechanisms. This study aimed to explore the diversity and physiological characteristics of rhizosphere bacteria associated with pineapple (Ananas comosus L. Merr) cultivated in Rimbo Panjang Village, Kampar, Riau. Soil samples were collected diagonally at a depth of 0–20 cm around the pineapple roots, and bacterial isolation was carried out using Nutrient Agar (NA) medium. The isolated colonies were characterized morphologically and physiologically through KOH, Gram staining, catalase, and oxidase tests. Six bacterial isolates (RN1–RN6) were successfully obtained, showing variations in colony morphology, including color, elevation, and edge structure. Physiological tests revealed that five isolates were Gram-positive and one isolate was Gram-negative, with all isolates showing positive catalase and negative oxidase activities. The dominance of Gram-positive bacteria suggests stable soil microbial conditions and potential functional roles in plant growth promotion and biological control. These findings provide preliminary information for further molecular identification and functional testing of rhizosphere bacteria as potential biofertilizers and bioprotectants in sustainable pineapple cultivation.